Jun 16, 2026
Dynamic Light Scattering (DLS) Analysis of ATG9A Vesicles
- Elisabeth Holzer1
- 1Laboratory of Sascha Martens, Max Perutz Labs, University of Vienna, Austria
Protocol Citation: Elisabeth Holzer 2026. Dynamic Light Scattering (DLS) Analysis of ATG9A Vesicles. protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvmbnm5g3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
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Created: July 31, 2025
Last Modified: June 16, 2026
Protocol Integer ID: 223860
Keywords: Dynamic Light Scattering, ATG9A Vesicles, analysis of atg9a vesicle, atg9a vesicle, dynamic light scattering, scattering analysis, dynamic light
Funders Acknowledgements:
Aligning Science Across Parkinson’s (ASAP)
Grant ID: ASAP-000350
DOC Fellowship (Austrian Academy of Sciences)
Abstract
This protocol details the dynamic light scattering analysis of ATG9A vesicles.
Materials
Instrument: DynaPro NanoStar (Wyatt Technology)
Cuvette: Quartz cuvette (compatible with DynaPro NanoStar)
Software: Dynamics 7.6.1.9 (Wyatt Technology)
Buffer:
o 20 millimolar (mM) HEPES, 7.4
o 150 millimolar (mM) NaCl
o 1 millimolar (mM) TCEP
Sample Preparation
Analyze the following conditions:
Buffer only (negative control).
MOCK vesicles (isolated from HAP1 wild-type cells not expressing tagged ATG9A).
ATG9A-containing vesicles (purified preparation).
Instrument Setup
Power on the DynaPro NanoStar and allow to equilibrate.
Load around 50 µL of sample into the quartz cuvette.
Data Acquisition
Perform measurements at 25 °C .
For each sample, acquire 10 consecutive measurements, each consisting of a 5-second acquisition.
Data Analysis
Analyze light scattering data using Dynamics 7.6.1.9 software (Wyatt Technology).
Use the globular protein model to interpret particle size distributions.