Sep 10, 2020

Public workspaceDural Cell Isolation and Culturing - Collection

  • 1University of California, San Francisco
  • Neurodegeneration Method Development Community
    Tech. support email: ndcn-help@chanzuckerberg.com
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Collection CitationAndrea Argouarch 2020. Dural Cell Isolation and Culturing - Collection. protocols.io https://dx.doi.org/10.17504/protocols.io.8m2hu8e
Manuscript citation:
Argouarch AR, Schultz N, Yang AC, Jang Y, Garcia K, Cosme CG, Corrales CI, Nana AL, Karydas AM, Spina S, Grinberg LT, Miller B, Wyss-Coray T, Abyzov A, Goodarzi H, Seeley WW, Kao AW, Postmortem Human Dura Mater Cells Exhibit Phenotypic, Transcriptomic and Genetic Abnormalities that Impact their Use for Disease Modeling. Stem Cell Reviews and Reports 18(8). doi: 10.1007/s12015-022-10416-x
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 22, 2019
Last Modified: September 10, 2020
Collection Integer ID: 29082
Abstract
Protocols included in this collection describe cell isolation from human dura mater, culturing, and banking.
Guidelines
All protocols described in this collection should be performed using sterile technique in a biosafety cabinet with appropriate PPE.

Materials
Relevant materials are listed in the protocols in this collection.
Files
Protocol
Icon representing the file Dural Cell Isolation
Name
Dural Cell Isolation
Version 1
, University of California, San Francisco
Andrea ArgouarchUniversity of California, San Francisco
Protocol
Icon representing the file Feeding 6wp
Name
Feeding 6wp
Version 1
, University of California, San Francisco
Andrea ArgouarchUniversity of California, San Francisco
Protocol
Icon representing the file Splitting p0 (6wp) to p1 (T75)
Name
Splitting p0 (6wp) to p1 (T75)
Version 1
, University of California, San Francisco
Andrea ArgouarchUniversity of California, San Francisco
Protocol
Icon representing the file Feeding T75, T150, and 6wp Backup
Name
Feeding T75, T150, and 6wp Backup
Version 1
, University of California, San Francisco
Andrea ArgouarchUniversity of California, San Francisco
Protocol
Icon representing the file Splitting p1 (1xT75) to p2 (2xT150)
Name
Splitting p1 (1xT75) to p2 (2xT150)
Version 1
, University of California, San Francisco
Andrea ArgouarchUniversity of California, San Francisco
Protocol
Icon representing the file Freezing/Cell Banking
Name
Freezing/Cell Banking
Version 1
, University of California, San Francisco
Andrea ArgouarchUniversity of California, San Francisco
Protocol
Icon representing the file Mycoplasma
Name
Mycoplasma
Version 1
, University of California, San Francisco
Andrea ArgouarchUniversity of California, San Francisco