Oct 04, 2023
  • Natalie L. Kendziorski1,2,
  • Tse-Yu Chen1,2,
  • Sara Farless1,2,
  • Sandra A. Allan3,
  • Chelsea T. Smartt1,2
  • 1Florida Medical Entomology Laboratory;
  • 2University of Florida;
  • 3United States Department of Agriculture
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Protocol CitationNatalie L. Kendziorski, Tse-Yu Chen, Sara Farless, Sandra A. Allan, Chelsea T. Smartt 2023. Dual-Choice Chamber Assay. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9p1j4g3e/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 04, 2023
Last Modified: October 04, 2023
Protocol  Integer ID: 88794
Keywords: mosquito responses to repellent, choice chamber assay system, choice chamber assay, modified system detects repellency, system detects repellency, mosquito response, laboratory assay, mosquito, repellency, repellent, movement of the mosquito, assay, essential oil, choice chamber system, laboratory
Funders Acknowledgements:
NSF Center for Arthropod Management Technologies IUCRC Phase II
Grant ID: AWD05009_MOD0030
Abstract
The protocol describes a laboratory assay to test the repellency of an essential oil using a modified dual-choice chamber assay system. The assay assesses the movement of the mosquito away from or towards the essential oil. The modified system detects repellency. Mosquito responses to repellent
compounds can be evaluated in a laboratory dual-choice chamber system.
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