License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: December 22, 2022
Last Modified: January 23, 2023
Collection Integer ID: 74375
Keywords: Plants, DToL, Darwin Tree of Life, RBGE, DNA barcoding, dtol taxon, dna barcoding, darwin tree, various plant taxa within the scope, darwin tree of life, various plant taxa, frozen plant tissue, whole genome sequencing, dtol, flash frozen plant tissue, genome, plant working group, sequencing, specific standard operating procedure, tissue sample, dna, procedure, standard operating procedure, whole genome, royal botanic garden, sop collection, place at the royal botanic garden, specimen
Abstract
This Standard Operating Procedure (SOP) collection contains guidance on how to process the various plant taxa within the scope of the Darwin Tree of Life (DToL) project. The guidance specifically refers to the tissue samples needed for DNA barcoding (which takes place at the Royal Botanic Garden Edinburgh (RBGE)) and outlines the flash frozen plant tissues required for whole genome sequencing (WGS), which takes place at the Wellcome Sanger Institute . Every specimen is submitted for DNA barcoding first before potentially being sent to the Wellcome Sanger Institute.
Guidelines
Use: These guides outline how to collect and prepare specimens for DNA barcoding, genome sequencing, and specimen vouchering when relevant. Different taxa may require different sampling, preparation, photography, storage techniques and processes.
Please see the Sanger Sample Submission SOP (link below) for general details on physical handling of specimens, including cold chain use, and collation of metadata for sample submission to Sanger.
Future plans for these SOPs: These SOPs will be reviewed on an ad hoc basis by the Plant Working Group to incorporate feedback from the community.
Raising issues: We are still developing best practice and elements of these SOPs are subject to change. If you would like to share any advice, comments, techniques or lessons learned, please add comments to the protocol or email [email protected].
