Nov 19, 2020

Public workspaceDry cell weight by centrifugation

DOI
dx.doi.org/10.17504/protocols.io.bkrbkv2n
  • 1Ronin Institute
Joao Vitor Molino
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DOI: dx.doi.org/10.17504/protocols.io.bkrbkv2n
Protocol Citation: Joao Vitor Molino 2020. Dry cell weight by centrifugation. protocols.io https://dx.doi.org/10.17504/protocols.io.bkrbkv2n
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 03, 2020
Last Modified: November 19, 2020
Protocol Integer ID: 41475
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Abstract
This protocols describe the steps required for dry cell weight measurements using microcentrifugal tubes.
Guidelines
Cell density for harvesting is important. The final mass weighted need to be inside the linear range of the balance.
All steps described in this protocol are intended to be conducted in a research laboratory.
Material
Material
  • Analytical balance with high precision (The higher the precision the better. For example a balance with a 0.1mg readability, could account to approximately 10% error alone in a measurement of 1mL sample of a culture at 1g/L)
  • Microcentrifugal tubes
  • Microcentrifuge
Tubes preparation
Tubes preparation
  1. Label microcentrifugal tubes
  2. Dry the tubes at Temperature90 °C , DurationOvernight
  3. Cool tubes at TemperatureRoom temperature for Duration00:30:00
  4. Record the weight of the tubes
Weighting the dry cell material
Weighting the dry cell material
  1. Harvest 2 mL of culture in a previously weighted tube
  2. Centrifuge the sample at Centrifigation20000 rcf, 25°C, 00:01:00
  3. Carefully remove the supernatant by pipetting
  4. Wash the cells with ddH20, and centrifuge the sample at Centrifigation20000 rcf, 25°C, 00:01:00
  5. Carefully remove the supernatant by pipetting
  6. Dry the tubes at Temperature90 °C , DurationOvernight
  7. Cool tubes at TemperatureRoom temperature for Duration00:30:00
  8. Record the weight of the tubes
  9. Subtract the initial tube weight to achieve the dry cell weight