This protocol describes how to make an egg dechorionator using available lab equipment before describing how to successfully use the egg dechorionator to remove the outer chorion layer from a Drosophila egg. Egg dechorionation allows cellular analysis, quantitative microinjection and the rearing of developing offspring in a gnotobiotic or axenic environment. This protocol is written with the use of dechorionated eggs for rearing fruit flies in a gnotobiotic or axenic environment in mind. Therefore sterile techniques are described. For cellular analysis and\/or quantitative microinjection the protocol can be followed but with a less stringent sterile procedure where unneccesary.