Oct 18, 2020

Public workspaceDouble digested

DOI
dx.doi.org/10.17504/protocols.io.bni6mche
  • 1South China University of Technology
Jiaxin Li
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DOI: dx.doi.org/10.17504/protocols.io.bni6mche
Protocol Citation: Jiaxin Li 2020. Double digested. protocols.io https://dx.doi.org/10.17504/protocols.io.bni6mche
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 18, 2020
Last Modified: October 18, 2020
Protocol Integer ID: 43326
Add the following reagents to a tube.
Total10ul
10 x Buffer1ul
Template（500ng）According to different template
EnzymeEach for 0.5ul
ddWaterAdd to 10ul
Pipette up and down thoroughly.
Put at 37℃ for about 2 hours (according to different enzymes).
Agarose Gel Electrophoresis
GroupDouble restriction digestSingle restriction digest 1Single restriction digest 2TemplateMarker
Enzyme-digested product10ul10ul10ul3ul（c）/
Theoretical Loading Buffer1.1ul1.1ul1.1ul0.4ul（c）/
Actual Loading Buffer2ul2ul2ul1ul/
Total 12ul12ul12ul4ul4ul