Mar 07, 2024

DOH Workshop Protocol Parts 1, 2 and 3 V.1

DOI
https://dx.doi.org/10.17504/protocols.io.q26g7pqpkgwz/v1
DOH Workshop Protocol Parts 1, 2 and 3
  • 1The Roslin Institute, University of Edinburgh
Bryan Wee
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DOI: https://dx.doi.org/10.17504/protocols.io.q26g7pqpkgwz/v1
Collection Citation: Vesa Qarkaxhija, Bryan Wee, Natalie Ring 2024. DOH Workshop Protocol Parts 1, 2 and 3. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7pqpkgwz/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this collection
Created: March 06, 2024
Last Modified: March 07, 2024
Collection  Integer ID: 96355
Keywords: purification of dsdna, low molecular weight dna, weight dna from gram, dsdna, libraries for the minion flow cell, negative bacterial culture, minion flow cell, nanopore, creation of nanopore, weight dna, dna, sequencing library, purification, sequencing, protocol, extraction
Funders Acknowledgements:
Royal Society
Grant ID: RGS\R1\231137
Abstract
This collection of protocols details the extraction of high-molecular-weight DNA from Gram-negative bacterial culture, purification of dsDNA, removing contaminants and low molecular weight DNA, and creation of Nanopore sequencing libraries for the MinION flow cell.
Files
Protocol
Icon representing the file DOH Workshop Protocol Part 1: Purification of High- Molecular Weight Genomic DNA from Gram-Negative Bacteria (MagAttract HMW DNA)
Name
DOH Workshop Protocol Part 1: Purification of High- Molecular Weight Genomic DNA from Gram-Negative Bacteria (MagAttract HMW DNA)
Version 1
Created by
Image of Bryan Wee, The University of Edinburgh
Bryan WeeThe University of Edinburgh
Protocol
Icon representing the file DOH Workshop Part 2: Promega Pronex protocol
Name
DOH Workshop Part 2: Promega Pronex protocol
Version 1
Created by
Image of Bryan Wee, The University of Edinburgh
Bryan WeeThe University of Edinburgh
Protocol
Icon representing the file DOH Workshop Protocol Part 3: Library preparation for Rapid Sequencing DNA V14 Barcoding kit (SQK-RBK114.24) with Pronex modification
Name
DOH Workshop Protocol Part 3: Library preparation for Rapid Sequencing DNA V14 Barcoding kit (SQK-RBK114.24) with Pronex modification
Version 1
Created by
Image of Bryan Wee, The University of Edinburgh
Bryan WeeThe University of Edinburgh