Feb 16, 2024

Public workspaceDo, Q. B. et al. (2023) Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation V.2

DOI
dx.doi.org/10.17504/protocols.io.x54v9dw61g3e/v2
  • Quyen Do1,2,3,
  • Humaira Noor1,2,3,
  • Ricardo Marquez Gomez1,2,3,
  • Kaitlyn M L Cramb1,2,3,
  • Bryan Ng1,2,
  • Ajantha Abbey1,2,
  • Naroa Ibarra-Aizpura1,2,
  • Maria-Claudia Caiazza1,2,3,
  • Parnaz Sharifi1,2,
  • Charmaine Lang1,2,3,
  • Dayne Beccano-Kelly1,2,
  • Jimena Baleriola4,5,
  • Nora Bengoa-Vergniory1,2,3,4,5,5,
  • Richard Wade-Martins1,2,3
  • 1Oxford Parkinson's Disease Centre and Department of Physiology, Anatomy and Genetics, University of Oxford, South Park Road, Oxford OX1 3QU, United Kingdom;
  • 2Kavli Institute for Neuroscience Discovery, University of Oxford, Dorothy Crowfoot Hodgkin Building, South Park Road, Oxford OX1 3QU, United Kingdom;
  • 3Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815, USA;
  • 4Achucarro Basque Center for Neuroscience, Leioa, Spain;
  • 5Ikerbasque - Basque Foundation for Science, Bilbao, Spain
Cláudia C. Mendes
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DOI: dx.doi.org/10.17504/protocols.io.x54v9dw61g3e/v2
External link: https://www.biorxiv.org/content/10.1101/2023.03.01.530566v1
Collection Citation: Quyen Do, Humaira Noor, Ricardo Marquez Gomez, Kaitlyn M L Cramb, Bryan Ng, Ajantha Abbey, Naroa Ibarra-Aizpura, Maria-Claudia Caiazza, Parnaz Sharifi, Charmaine Lang, Dayne Beccano-Kelly, Jimena Baleriola, Nora Bengoa-Vergniory, Richard Wade-Martins 2024. Do, Q. B. et al. (2023) Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9dw61g3e/v2Version created by Cláudia C. Mendes
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 16, 2024
Last Modified: February 16, 2024
Collection Integer ID: 95342
Funders Acknowledgements:
Aligning Science Across Parkinson’s (ASAP)
Grant ID: ASAP-020370
Abstract
This collection contains ten protocols detailing methods used in Do, Q. B. et al. (2023) Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation.
Files
Protocol
Icon representing the file Expansion and maintenance of human induced pluripotent stem cells (iPSCs)
Name
Expansion and maintenance of human induced pluripotent stem cells (iPSCs)
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Custom open-chamber microfluidic fabrication
Name
Custom open-chamber microfluidic fabrication
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Differentiation of human Dopamine Neurons (DaNs) from induced pluripotent stem cells (iPSCs)
Name
Differentiation of human Dopamine Neurons (DaNs) from induced pluripotent stem cells (iPSCs)
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
Name
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
Name
Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
Version 1
,
Quyen Do
Protocol
Icon representing the file Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)
Name
Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Visualisation and quantification of dendritic spines in cultured human Medium Spiny Neurons (MSNs)
Name
Visualisation and quantification of dendritic spines in cultured human Medium Spiny Neurons (MSNs)
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Whole-cell patch-clamping of cultured human neurons
Name
Whole-cell patch-clamping of cultured human neurons
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Measuring dopamine release in human-derived iPSCs using High-Performance Liquid Chromatography (HPLC) coupled to Electrochemical Detection (ECD)
Name
Measuring dopamine release in human-derived iPSCs using High-Performance Liquid Chromatography (HPLC) coupled to Electrochemical Detection (ECD)
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Quantification of glutamate released from human induced pluripotent stem cells (iPSC) derived cortical neurons (CNs)
Name
Quantification of glutamate released from human induced pluripotent stem cells (iPSC) derived cortical neurons (CNs)
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford