Jul 22, 2025

DNAMA VSVC Guidebook V.3

DOI
https://dx.doi.org/10.17504/protocols.io.g5z7by79p
  • 1FUNDIS;
  • 2Dikarya LLC;
  • 3Cal State East Bay;
  • 4North American
Harte Singer
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DOI: https://dx.doi.org/10.17504/protocols.io.g5z7by79p
External link: http://www.namyco.org
Protocol Citation: Harte Singer 2025. DNAMA VSVC Guidebook. protocols.io https://dx.doi.org/10.17504/protocols.io.g5z7by79pVersion created by Harte Singer
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 22, 2025
Last Modified: July 22, 2025
Protocol  Integer ID: 223007
Keywords: Mycology, DNA Barcoding, Fungi, Nanopore, ITS, Community Science, fungal, oxford nanopore technology, sequencing platform, guidebook, barcode data, dnama vsvc guidebook, information on haplotype, haplotype, ont
Funders Acknowledgements:
California Institute for Biodiversity
Grant ID: 89685
Abstract
A guidebook for learning how to analyze and validate fungal nrITS barcode data generated by Oxford Nanopore Technologies (ONT) MinION sequencing platform using free software and online platforms. Version 3 22-July-2025

Summary of changes: Added information on haplotype phasing including a video and a slide show. Added a link to the cloud version of the document. Added link to definitions of different types, i.e. holotype.
Attachments
Icon for _Validation Protocol for VSVC.pdf
_Validation Protocol...
675KB
Materials
A modern computer with an internet connection
UGENE - https://ugene.net/
MycoMap.com Account
iNaturalist account