Sep 08, 2020

Public workspaceDNA Quantification -- CHEM 584

 Forked from DNA Concentration Measurement (Protocol for Thermo Scientific NanoDrop™ 1000 Spectrophotometer)
DOI
dx.doi.org/10.17504/protocols.io.bk4xkyxn
  • 1Wageningen University;
  • 2Brigham Young University
Ken Christensen
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DOI: dx.doi.org/10.17504/protocols.io.bk4xkyxn
Protocol Citation: Alba Balletbó, Ken Christensen 2020. DNA Quantification -- CHEM 584. protocols.io https://dx.doi.org/10.17504/protocols.io.bk4xkyxn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: September 08, 2020
Last Modified: September 08, 2020
Protocol Integer ID: 41847
Abstract
Protocol adapted from the NanoDrop Spectrophotometer User’s Manual.
Materials
TE Buffer or MQ Water
DNA Sample
Start a new experiment using the Nanodrop touchscreen.


Click on the corresponding application module (e.g., dsDNA).
Open the sampling arm and load a blank sample (e.g., TE Buffer, MQ Water, etc.).
Amount2 µL

Close the sampling arm on the machine to cover the blank sample.
Click "OK" to read the blank if the Nanodrop is not already in automatic mode.
Open the sampling arm and clean the blank off the upper and lower pedestals using a Kim Wipe.
Load your DNA sample and close the sampling arm.
Amount2 µL

Click "Measure sample" if the Nanodrop is not in automatic mode.
Take a picture of the screen that shows your results and the corresponding spectra for your records.
Clean the nucleic acid sample off of the upper and lower pedestals using a Kim Wipe.
Repeat for all samples.
Close program.