Sep 26, 2019

Public workspaceDNA Gel Electrophoresis

DOI
dx.doi.org/10.17504/protocols.io.7pshmne
  • 1National University of Singapore
NUS iGEM
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DOI: dx.doi.org/10.17504/protocols.io.7pshmne
Protocol Citation: NUS iGEM 2019. DNA Gel Electrophoresis. protocols.io https://dx.doi.org/10.17504/protocols.io.7pshmne
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 26, 2019
Last Modified: September 12, 2023
Protocol Integer ID: 28114
Materials
STEP MATERIALS
ReagentDNA Gel Loading Dye (6X)Thermo Fisher ScientificCatalog #R0611
Protocol materials
ReagentDNA Gel Loading Dye (6X)Thermo Fisher ScientificCatalog #R0611
ReagentDNA Gel Loading Dye (6X)Thermo Fisher ScientificCatalog #R0611
Load Amount10 µL of 6X loading dye into Amount50 µL of P product

ReagentDNA Gel Loading Dye (6X)Thermo Fisher ScientificCatalog #R0611


Mix and load entire volume into 1% agarose gel
Load Amount10 µL of 1kb or 100bp ladder

Run gel at 120V for Duration00:20:00

Visualize band under blue light transilluminator