Jan 14, 2026
DNA extraction with Modified GenoLyse kit
- Hubert Senanu1,
- Micheal Frimpong1
- 1Kumasi Centre for Collaborative Research, Kwame Nkrumah University of Science and Technology
Protocol Citation: Hubert Senanu, Micheal Frimpong 2026. DNA extraction with Modified GenoLyse kit. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ld1myol5b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 13, 2026
Last Modified: January 14, 2026
Protocol Integer ID: 238535
Keywords: dna extraction with modified genolyse kit, bacterial dna from patient specimen, dna extraction, efficient dna extraction, bacterial dna, modified genolyse kit, genolyse kit, dna, test procedure, extraction, whole test procedure, isolate, specimen, simple procedure, procedure, patient specimen, standard operating procedure, culture sample
Abstract
This document describes the standard operating procedure for a fast and easy way to isolate bacterial DNA from patient specimens and culture samples using the GenoLyse kit. The simple procedure allows for a fast and efficient DNA extraction in less than 20 minutes. Since the DNA extraction only takes a short amount of time, the whole test procedure is reduced. The procedure requires minimal instrumentation.
Materials
- Hain Genolyze kit (Hain, Germany)
- Phosphate buffer solution
- 1.5 ml/ 2 ml DNase/RNase free centrifuge tubes (Eppendorf, Germany)
- 100 μl, 1000 μl pipette filter tips, DNase/Rnase free (Biozym, Germany).
- Mini-Centrifuge
- Dry block heater
- Vortexer
- Tube racks
Troubleshooting
General considerations
This document describes the standard operating procedure for a fast and easy way to isolate bacterial DNA from patient specimens and culture samples using the GenoLyse kit. The simple procedure allows for a fast and efficient DNA extraction in less than 20 minutes. Since the DNA extraction only takes a short amount of time, the whole test procedure is reduced. The procedure requires minimal instrumentation.
Preparations
GenoLyse kits should be stored at 2-8 °C. All other solutions could be stirred at room temperature.
Procedure
Elute samples into GenoLyse buffer (250 µl for swabs or 100 µl for FNA).
Vortex vigorously for 20 sec.
Incubate samples for 10 min at 95 °C (Remove swabs before incubation).
Add equal volume of neutralization buffer.
Centrifuge at high speed for 5 mins.
The extracted DNA (supernatant) may be directly used for amplification or stored at -20 °C.
Protocol references
Frimpong M, Ahor HS, Sakyi SA, Agbavor B, Akowuah E, Phillips RO. Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients. Diagnostics (Basel). 2019;9(4). Epub 20191126. doi: 10.3390/diagnostics9040204. PubMed PMID: 31779247; PubMed Central PMCID: PMCPMC6963521.