Feb 12, 2024
DNA extraction protocol for saliva samples obtained from Omigene oral OM device using the Qiagen QiaAMP Powerfecal DNA kit for shotgun metagenomic sequencing
- Victoria Meslier1,
- Aymeric David1,
- Christian MORABITO2,
- Mathieu Almeida3
- 1Université Paris Saclay, INRAE MetaGenoPolis, 78350 Jouy-en-Josas, France;
- 2INRAE;
- 3INRAE MetaGenoPolis
Protocol Citation: Victoria Meslier, Aymeric David, Christian MORABITO, Mathieu Almeida 2024. DNA extraction protocol for saliva samples obtained from Omigene oral OM device using the Qiagen QiaAMP Powerfecal DNA kit for shotgun metagenomic sequencing. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyjyrplx9/v1
Manuscript citation:
Meslier, V.; Menozzi, E.; David, A.; Morabito,
C.; Lucas Del Pozo, S.; Famechon, A.; North, J.; Quinquis, B.; Koletsi, S.;
Macnaughtan, J.; et al. Evaluation of an Adapted Semi-Automated DNA Extraction
for Human Salivary Shotgun Metagenomics. Biomolecules 2023, 13, 1505.
https://doi.org/10.3390/biom13101505
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 08, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 94923
Keywords: ASAPCRN
Funders Acknowledgements:
Agence Nationale de la Recherche
Grant ID: ANR-11-DPBS-0001
Aligning Science Across Parkinson's
Grant ID: ASAP-000420
Abstract
This protocol describes the procedure for DNA extraction of saliva samples using the Omnigene OM device and the Qiagen QiaMP Powerfecal DNA PRO kit for shotgun metagenomic sequencing.
Attachments
Guidelines
NA
Materials
see attached document
Safety warnings
see attached protocol
Ethics statement
NA
Before start
visit the protocols.io for saliva sampling : https://dx.doi.org/10.17504/protocols.io.n92ldm8qol5b/v1
Salivary Collection Using OMNIgene ORAL OM 501 Device
Salivary Collection Using OMNIgene ORAL OM 501 Device
Visit the protocols.io for saliva sampling : https://dx.doi.org/10.17504/protocols.io.n92ldm8qol5b/v1
Sample pre-processing
Sample pre-processing
Heat the whole OMNIgene Oral OM 501 tubes into an incubator at 50 °C for 2 h.
DNA extraction QIAGEN QIAamp Power Fecal Pro
DNA extraction QIAGEN QIAamp Power Fecal Pro
Available at https://www.qiagen.com/
Protocol references
Meslier, V.; Menozzi, E.; David, A.; Morabito, C.; Lucas Del Pozo, S.; Famechon, A.;
North, J.; Quinquis, B.; Koletsi, S.; Macnaughtan, J.; et al. Evaluation of an
Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics.
Biomolecules 2023, 13, 1505. https://doi.org/10.3390/biom13101505