This protocol is a modified version of the MoBio PowerPlant Pro DNA extraction kit for plant and lichen tissues that have been stored in CTAB buffer. Prior to being placed in CTAB, photosynthetic tissues from plants and lichens were surface-sterilized with sequential washes in 95% EtOH, 0.5% sodium hypochlorite, and 70% EtOH and air dried under sterile conditions. Twenty-four tissue pieces per host were placed in 750 uL of CTAB buffer. Four tubes per host individual were sampled for a total of 96 tissue pieces.