Nov 14, 2023

Public workspaceDNA barcoding SOPs for the Darwin Tree of Life Project

DOI
dx.doi.org/10.17504/protocols.io.261ged91jv47/v1
  • 1Natural History Museum London;
  • 2Marine Biological Association;
  • 3Royal Botanic Gardens of Edinburgh;
  • 4University of Oxford
  • Darwin Tree of Life
Jordan Beasley
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DOI: dx.doi.org/10.17504/protocols.io.261ged91jv47/v1
Collection Citation: Jordan Beasley, Rebekka Uhl, Laura L Forrest, David Bell, Michelle Hart, Rebecca Yahr, Estelle Kilias 2023. DNA barcoding SOPs for the Darwin Tree of Life Project. protocols.io https://dx.doi.org/10.17504/protocols.io.261ged91jv47/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: November 13, 2023
Last Modified: November 14, 2023
Collection Integer ID: 90854
Abstract
This collection of protocols describe the standard operating procedures for DNA barcoding in the Darwin Tree of Life project.
The SOPs cover barcoding of macroalgae and marine protists, fungi and lichens at the Marine Biological Association, protists at the University of Oxford, plants and lichens at the Royal Botanic Gardens of Edinburgh and animals at the Natural History Museum of London.

Files
Protocol
Icon representing the file MBA DToL DNA barcoding of macroalgae and marine protists, fungi and lichens – detailed Standard Operating Procedure
Name
MBA DToL DNA barcoding of macroalgae and marine protists, fungi and lichens – detailed Standard Operating Procedure
Version 1
,
Rebekka Uhl
Protocol
Icon representing the file DNA Barcoding Protocol for Darwin Tree of Life at The Natural History Museum of London
Name
DNA Barcoding Protocol for Darwin Tree of Life at The Natural History Museum of London
Version 1
, Natural History Museum London
Jordan BeasleyNatural History Museum London
Protocol
Icon representing the file Taxon Group Protists: Barcoding
Name
Taxon Group Protists: Barcoding
Version 1
, University of Oxford
Estelle KiliasUniversity of Oxford
Protocol
Icon representing the file DNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Sample Data
Name
ForkDNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Sample Data
Version 1
, Royal Botanic Garden Edinburgh
Laura L ForrestRoyal Botanic Garden Edinburgh
Protocol
Icon representing the file DNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Lab methods: DNA extraction
Name
ForkDNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Lab methods: DNA extraction
Version 1
, Royal Botanic Garden Edinburgh
Laura L ForrestRoyal Botanic Garden Edinburgh
Protocol
Icon representing the file DNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Sanger Sequence Data
Name
ForkDNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Sanger Sequence Data
Version 1
, Royal Botanic Garden Edinburgh
Laura L ForrestRoyal Botanic Garden Edinburgh
Protocol
Icon representing the file DNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Lab methods: PCR and Sequencing
Name
ForkDNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Lab methods: PCR and Sequencing
Version 1
, Royal Botanic Garden Edinburgh
Laura L ForrestRoyal Botanic Garden Edinburgh