Oct 07, 2025
Differentiation-hiPSC-derived astrocyte
- xiaoxi 1
- 1Department of Psychiatry, School of Medicine, Yale University, New Haven, CT, 06511
- Xi's
Protocol Citation: xiaoxi 2025. Differentiation-hiPSC-derived astrocyte. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl88w7rl2w/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 06, 2025
Last Modified: October 07, 2025
Protocol Integer ID: 229152
Keywords: derived astrocyte, homogeneous populations of induced astrocyte, induced astrocyte, overexpression of the transcription factors sox9, transcription factors sox9, astrocyte this protocol details step, nfib in human pluripotent stem cell, human pluripotent stem cell, astrocyte
Funders Acknowledgements:
Xi Xiao
Abstract
This protocol details steps for overexpression of the transcription factors SOX9 and NFIB in human pluripotent stem cells rapidly and efficiently yields homogeneous populations of induced astrocytes. This protocol takes 21 days.
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