Aug 29, 2024
DFP-induced mitophagy experiments
- Elias Adriaenssens1
- 1Sascha Martens lab, University of Vienna, Max Perutz Labs - Vienna
External link: https://doi.org/10.1038/s41556-025-01712-y
Protocol Citation: Elias Adriaenssens 2024. DFP-induced mitophagy experiments. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvw11m9lmk/v1
Manuscript citation:
Adriaenssens, E., Schaar, S., Cook, A.S.I. et al. Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery. Nat Cell Biol 27, 1272–1287 (2025). https://doi.org/10.1038/s41556-025-01712-y
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 23, 2024
Last Modified: August 29, 2024
Protocol Integer ID: 101114
Keywords: ASAPCRN, induced mitophagy experiment, mitophagy experiments this protocol detail, mitophagy experiment, dfp
Funders Acknowledgements:
Aligning Science Across Parkinson’s (ASAP)
Grant ID: ASAP-000350
Marie Skłodowska-Curie MSCA Postdoctoral fellowship
Grant ID: 101062916
Abstract
This protocol details the DFP-induced mitophagy experiments.
Materials
Deferiprone (DFP) (379409, Sigma Aldrich)
3-Hydroxy-1,2-dimethyl-4(1H)-pyridoneMerck MilliporeSigma (Sigma-Aldrich)Catalog #379409
Troubleshooting
DFP-induced mitophagy
2d
To induce mitophagy,
- Treat the cells for 24:00:00 with 1 millimolar (mM) Deferiprone (DFP) (379409, Sigma Aldrich), an iron chelator that mimics hypoxic conditions through stabilization of the transcription factor HIF1α and subsequent upregulation of NIX and BNIP3.
1d
Freshly dissolve the compound in sterile dH2O on the day itself and boil for 00:01:00 at 95 °C to dissolve the compound.
1m