Mar 05, 2025
Dewaxing and rehydration of paraffin sections for histology
- Samson Dowland1
- 1CPC Histology Facility, The University of Sydney
- The University of Sydney
Protocol Citation: Samson Dowland 2025. Dewaxing and rehydration of paraffin sections for histology. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj9kk5lk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 04, 2025
Last Modified: March 05, 2025
Protocol Integer ID: 123815
Keywords: histology paraffin, rehydration of paraffin section, tissue sections before histological staining, histological staining, paraffin section, staining with aqueous solution, staining solution, rehydration of section, tissue section, method for deparrafinisation, deparrafinisation, rehydration, tissue
Abstract
Paraffin must be removed from tissue sections before histological staining. This protocol describes the method for deparrafinisation and rehydration of sections to facilitate staining with aqueous solutions. The protocol may be shortened as appropriate if using an alcoholic staining solution.
Protocol materials
XylenePOCD ScientificCatalog #XYL5P
EthanolPOCD ScientificCatalog #ETHABS20M
EthanolHurst ScientificCatalog #EIMS95-5L
EthanolPOCD ScientificCatalog #ETHABS70WV5
Troubleshooting
Xylene100 % (v/v) XylenePOCD ScientificCatalog #XYL5P
10m
Xylene100 % (v/v) XylenePOCD ScientificCatalog #XYL5P
10m
100% Ethanol100 % (v/v) EthanolPOCD ScientificCatalog #ETHABS20M
2m
100% Ethanol100 % (v/v) EthanolPOCD ScientificCatalog #ETHABS20M
2m
95% Ethanol95 % (v/v) EthanolHurst ScientificCatalog #EIMS95-5L
2m
95% Ethanol95 % (v/v) EthanolHurst ScientificCatalog #EIMS95-5L
2m
70% Ethanol70 Mass / % volume EthanolPOCD ScientificCatalog #ETHABS70WV5
2m
Tap Water
2m