1Unidad Multidisciplinaria de Docencia e Investigación. Facultad de Ciencias, Universidad Nacional Autónoma de México. Puerto de abrigo s/n, Sisal, Yucatán, México. C. P. 97356;
2Unidad de Química en Sisal. Facultad de Química, Universidad Nacional Autónoma de México, Puerto de abrigo s/n, Sisal, Yucatán, México. C. P. 97356
Collection Citation: Daisy Pineda-Suazo, Wendy Escobedo-Hinojosa, Pedro Gallardo, Carlos Rosas 2025. Development and validation of a static in vitro digestion system for Octopus maya. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp92z5vzp/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: January 20, 2025
Last Modified: March 06, 2025
Collection Integer ID: 118837
Keywords: Octopus maya, Enzyme activity, Gastric juice, Cephalopod digestion, In vitro digestion system, digestion system for octopus maya, cephalopod gastrointestinal simulation system, digestive physiology in octopus maya, advancements in cephalopod culture, physiological digestion, digestion system, protein digestion value, cephalopod culture, protein digestion, digestive physiology, using gastric juice, significant differences in protein digestion, understanding digestive process, diet formulation, optimizing diet formulation, octopus maya, vivo trial, traditional in vivo method, novel in vitro model, vivo method, sustainability in aquaculture, vitro model, aquaculture, protein, vivo
Funders Acknowledgements:
Secretaría de Ciencia, Humanidades, Tecnología e Innovación
Grant ID: PRONAII-2024-70
Universidad Nacional Autónoma de México
Grant ID: PAPIIT IN203022
Universidad Nacional Autónoma de México
Grant ID: PROGRAMA DE BECAS POSDOCTORALES
Abstract
The Cephalopod Gastrointestinal Simulation System (CGSS) was developed and validated as a novel in vitro model to study digestive physiology in Octopus maya. This system replicates physiological digestion using gastric juice from O. maya and offers an ethical, cost-effective alternative to traditional in vivo methods.
The CGSS demonstrated high concordance with in vivo trials, with no significant differences in protein digestion (p > 0.05). For instance, protein digestion values were 0.567 ± 0.012 mg/mL (in vitro) and 0.562 ± 0.035 mg/mL (in vivo), confirming the system’s reliability. The CGSS provides a practical tool for optimizing diet formulations, including the use of alternative, sustainable ingredients. By reducing reliance on live animals, the system aligns with ethical research practices and promotes sustainability in aquaculture. This innovative model supports advancements in cephalopod culture, offering reliable, scalable solutions for improving productivity and understanding digestive processes in O. maya and other species.
