This protocol explains how to use serial dilutions of sample protein to determine the linear ranges of detection for a target and internal loading control, and choose an appropriate amount of sample to load for QWB analysis. Follow the instructions in this protocol if total protein staining of the membrane will be used as the internal loading control for QWB normalization. For normalization with a housekeeping protein, see https://www.protocols.io/view/determining-the-linear-range-for-a-target-protein-g77bzrn. Note: This protocol is intended for use with near-infrared fluorescent Western blots.