Aug 25, 2020
Determination of C4 concentration by the Mancini test.
- 1University of the West Indies St. Augustine
- University of the West Indies
- angel.vaillant@sta.uwi.edu
Protocol Citation: Angel A Justiz-Vaillant 2020. Determination of C4 concentration by the Mancini test.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj95kr86
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 24, 2020
Last Modified: August 25, 2020
Protocol Integer ID: 40989
An appropriate anti-C4 antiserum (antibody) is poured in the center well of an agar-containing plate.
Carefully circular wells are cut and detached from the plates.
A series of standards containing known concentrations of C4 are placed in separate wells, while “unknown” human serum samples and control are placed in other wells.
A ring of precipitate forms in the area of optimal antigen-antibody concentration, meaning anti-C4 - C4 reaction as the antigen diffuses radially.
The diameters of the rings are measured and perceived normally in 48-72 hours.
Finally, a standard curve is developed using the ring diameters of the standards versus the concentrations.
A curve is then used to plot the concentration of the control and unknown C4 samples.