Collection Citation: Joseph Patterson, Allyson Cole-Strauss, John Beck, Caryl Sortwell, Jack Lipton 2020. Detection of SARS-Cov2 Without High Demand Reagents (Singleplex Assays). protocols.io https://dx.doi.org/10.17504/protocols.io.be8sjhwe
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 16, 2020
Last Modified: December 10, 2020
Collection Integer ID: 35826
Keywords: COVID19, SARS-Cov2 Detection, Method Using Limited Resources
Abstract
In the United States, access to testing for the novel coronavirus (SARS-Cov2) is severely limited. Arguably, the PCR based tests are the most reliable when it comes to detecting the virus. Critical reagents for these tests, however, are in short supply. Our group has worked to identify and test alternative reagents and supplies that are not in high demand by clinical labs. We have adapted a more traditional approach to isolating RNA does not use a kit. RNA isolated can be used in traditional quantitative PCR or droplet digital PCR, which has been shown to be ~500 times more sensitive.
Guidelines
Samples should be processed for RNA extraction (at least up until they can be frozen at -80 ºC) within 48 hours of collection.
Safety warnings
Human samples should be handled with care, and sample prepartion performed in at least a BSL-2 lab.
