Mar 21, 2023

Public workspaceDeleidi Lab | Protocols | Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism

 Forked from Deleidi Lab | Protocols | "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly
DOI
https://dx.doi.org/10.17504/protocols.io.rm7vzbejrvx1/v1
  • michela.deleidi 1,
  • Maria Jose Perez J.1,
  • Hariam Raji1,
  • Federico Bertoli1,
  • Pascale Baden1
  • 1German Center for Neurodegenerative Diseases (DZNE), Tübingen, 72076 Germany
Federico Bertoli
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DOI: https://dx.doi.org/10.17504/protocols.io.rm7vzbejrvx1/v1
Collection Citation: michela.deleidi , Maria Jose Perez J., Hariam Raji, Federico Bertoli, Pascale Baden 2023. Deleidi Lab | Protocols | Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzbejrvx1/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: March 21, 2023
Last Modified: March 21, 2023
Collection Integer ID: 79098
Keywords: ASAPCRN, glucocerebrosidase, mitochondria, energy metabolism collection of protocol, energy metabolism collection, energy metabolism, team deleidi
Abstract
Collection of protocols of Team Deleidi used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism"
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Files
Protocol
Name
Plasmid-reprogramming of human fibroblasts
Version 1
, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Sequencing of construct
Version 1
, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Complex I activity assay
Version 1
, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Single cell dissociation of brain organoids
Version 1
, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Single cell analysis of iPSC-derived midbrain organoids
Version 1
, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
In vitro GCase activity assay (total cell lysate)
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Midbrain-like Organoids generation from hiPSCs
Version 1
, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163