Oct 01, 2025

Public workspaceDeleidi Lab | Collection of Protocols | The mitochondrial unfolded protein response disrupts neuronal–glial communication and promotes senescence in human microglia

  • Maria Jose Perez J.1,2,
  • michela.deleidi 1,2,
  • Alicia Lam1,
  • Christin Weissleder1,2,
  • Federico Bertoli1,2,
  • Hariam Raji1,2
  • 1Mechanisms and Therapy of Genetic Brain Diseases, Institut Imagine, Paris, France;
  • 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815
  • Team Deleidi
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Collection CitationMaria Jose Perez J., michela.deleidi , Alicia Lam, Christin Weissleder, Federico Bertoli, Hariam Raji 2025. Deleidi Lab | Collection of Protocols | The mitochondrial unfolded protein response disrupts neuronal–glial communication and promotes senescence in human microglia. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv5kbm4v1b/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: September 29, 2025
Last Modified: October 01, 2025
Collection Integer ID: 228453
Keywords: promotes senescence in human microglia collection, promotes senescence in human microglia, human microglia, human microglia collection, glial communication, mitochondrial, promotes senescence, team deleidi, protocols of team deleidi
Funders Acknowledgements:
Aligning Science Across Parkinson’s
Grant ID: ASAP-000420
Michael J. Fox Foundation for Parkinson’s Research (MJFF)
Grant ID:
ERC CoG
Grant ID: 101003329
DFG-ANR
Grant ID: 490761034
EU Joint Program—Neurodegenerative Disease Research (JPND) project GBA-PaCTS
Grant ID: 01ED2005B
nnovative Minds Program–Foundation Deutsche Demenzhilfe
EMBO Long term postdoctoral fellowship
Grant ID: ALTF 1013-2019
Becas Chile-ANID
Grant ID: 74190073
European Union’s Horizon Europe Research and Innovation Program through the MSCA–Doctoral Network NADIS
Grant ID: 101073251
Abstract
Collection of protocols of Team Deleidi used in the publication :"The mitochondrial unfolded protein response disrupts neuronal–glial communication and promotes senescence in human microglia"
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Files
Protocol
Name
Targeted LC‒MS metabolomics analyses
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Quantitative RT-PCR
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
NAD consumption assay
Version 1
, ASAP
Federico BertoliASAP
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Drug treatments
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
GSEA
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Mouse studies
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Generation of human iPSC-derived tricultures
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Immunofluorescence staining and image analysis
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Bulk RNA-seq and data analysis
Version 1
, ASAP
Federico BertoliASAP
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Name
α-synuclein PFF treatment
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Generation of human iPSC-derived cortical neurons, astrocytes, and microglia
Version 1
, ASAP
Federico BertoliASAP
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LONP1 knockdown in iPSC-derived microglia
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Cell Cycle Analysis by Flow Cytometry
Version 1
, ASAP
Federico BertoliASAP
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Name
Untargeted MS-based lipidomic analyses
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Nuclear senescence scoring
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Phagocytosis assay
Version 1
, ASAP
Federico BertoliASAP
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Name
Functional assays via live‒cell imaging
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Multiplex cytokine array analysis
Version 1
, ASAP
Federico BertoliASAP
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Name
Mitochondrial isolation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Western blot
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
β-Galactosidase staining
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Single-cell sequencing data analysis
Version 1
, ASAP
Federico BertoliASAP
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Name
Single-cell transcriptome library preparation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Human iPSC-derived MgBr assembloids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
ATF5-inducible overexpressing human iPSCs
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Autophagic flux analysis
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Measurement of protein aggregation with PROTEOSTAT
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Human iPSCs
Version 1
, ASAP
Federico BertoliASAP