May 08, 2025
Version 1
DeckLayout: automated indexing, purification and quantification of DNA libraries V.1
- Julia Zorn1
- 1Max Planck Institute for Evolutionary Anthropology
- MPI EVA Ancient DNA Core Unit
Document Citation: Julia Zorn 2025. DeckLayout: automated indexing, purification and quantification of DNA libraries. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbqxengpk/v1
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: April 28, 2025
Last Modified: May 08, 2025
Document Integer ID: 183633
Keywords: quantification of dna library, dna library, purification deck layout for hamilton workstation, purification deck layout, purification, overview of the library indexing, library indexing, hamilton workstation, automated indexing, existing ml star system, ml star system, decklayout
Funders Acknowledgements:
Max Planck Society
Grant ID: -
Abstract
This document provides an overview of the library indexing and purification deck layout for Hamilton workstations. It is intended to help users in setting up or modifying their existing ML STAR systems. Users are advised to consult Hamilton Company to acquire the necessary carriers, modules, inserts, and accessories required for proper system configuration.
Troubleshooting
Deck layout: automated indexing and purification of DNA libraries
Hamilton work station - NGS STAR MOA (custom-specific configuration: ML STAR with 8 independent channels, MPH96, 5 HHS, 3 CPAC). Please pay attention to build in adapters on Hamilton Heater Shakers (HHS). Required add-ons are: iSWAP Plate Handler (for full-deck plate transport), MPH Extension Left Standard (for usage of MPH96) and the ODTC (for on-deck thermal cycling of 96 well plates).
When setting up a Hamilton workstation, please consult Hamilton Company to obtain the required carriers, modules, inserts, and other accessories. The following documentation provides an overview of the indexing and purification deck layout, designed to assist you in setting up or modifying your existing ML STAR system.
Deck layout: Indexing PCR setup - running protocol and Hamilton system will give further instructions for usage of inserts and adapters.
Picture of empty deck (indexing PCR setup).
Picture of empty deck (indexing PCR setup).
Deck layout: Purification setup - running protocol and Hamilton system will give further instructions for usage of inserts and adapters.
Picture of empty deck (Purification setup).
Picture of empty deck (Purification setup).
Tube adapter for 16 x 2 ml tubes and 8 x 5 ml tubes for CPAC, supplier: Hamilton company (Hamilton APE solution).
Plate adapter for 96 well PCR plates for CPAC, supplier: Hamilton company.
Plate adapter for 384 well PCR plates for CPAC, supplier: Hamilton company.
Add-on option: trough lid for 60 ml reservoirs, supplier: Hamilton company (Hamilton APE solution).