Aug 05, 2020
De-salting of tryptic digested peptides
- 1domnic colvin
- UM-DAE Centre for Excellence in Basic Sciences
Document Citation: avinash.kale 2020. De-salting of tryptic digested peptides. protocols.io https://dx.doi.org/10.17504/protocols.io.bjdqki5w
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: August 05, 2020
Last Modified: August 05, 2020
Document Integer ID: 40080
De-salting of tryptic digested peptides
The desalting of tryptic digested samples were optimized and performed using octadecyl carbon chain (C18) pipette tips (ZipTip® Pipette Tips, Millipore). Briefly, for the C18 tips, the desalting pipette tip was pre-equilibrated using a solution of 50% Acetonitrile (SRL) in LC-MS grade water and then equilibrated twice in 0.1% formic acid in LC-MS water. After carefully and gently pipetting the entire sample up and down for at least 10 times, the membrane was washed with 0.1% formic acid in LC-MS water. The peptides were eluted in 70% acetonitrile in LC-MS grade water and vacuum dried and stored for further analysis(26).