Jun 30, 2025

Public workspaceDam&ChIC collection: Methods developed and implemented by Kefalopoulou et al., 2025

Nature Cell Biology
DOI
https://dx.doi.org/10.17504/protocols.io.6qpvrkdbplmk/v1
  • Samy Kefalopoulou1,
  • Peter Zeller1,2
  • 1Hubrecht Institute for Developmental Biology and Stem Cell Research;
  • 2Aarhus University, Department of Molecular Biology and Genetics
  • Dam&ChIC
Samy Kefalopoulou
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DOI: https://dx.doi.org/10.17504/protocols.io.6qpvrkdbplmk/v1
External link: https://doi.org/10.1038/s41556-025-01687-w
Collection Citation: Samy Kefalopoulou, Peter Zeller 2025. Dam&ChIC collection: Methods developed and implemented by Kefalopoulou et al., 2025. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvrkdbplmk/v1
Manuscript citation:
Samy Kefalopoulou, Pim M. J. Rullens, Kim L. de Luca, Sandra S. de Vries, Tessy Korthout, Alexander van Oudenaarden, Peter Zeller, Jop Kind (2025) Retrospective and multifactorial single-cell profiling reveals sequential chromatin reorganization during X inactivation.Nature Cell Biology doi: 10.1038/s41556-025-01687-w
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 15, 2025
Last Modified: June 30, 2025
Collection Integer ID: 126682
Keywords: such as sortchic, developed method, sortchic, detailed protocol, method, collection, dam
Abstract
This collection includes a detailed protocol for Dam&ChIC, developed by Kefalopoulou et al., 2025.
Additionally, it includes previously developed methods that were implemented in the study, such as sortChIC and scDam&T-seq.
Troubleshooting
Files
Protocol
Name
Dam&ChIC
Version 1
, Hubrecht Institute for Developmental Biology and Stem Cell Research
Samy KefalopoulouHubrecht Institute for Developmental Biology and Stem Cell Research
Protocol
Name
Dam&ChIC in bulk
Version 1
, Hubrecht Institute for Developmental Biology and Stem Cell Research
Samy KefalopoulouHubrecht Institute for Developmental Biology and Stem Cell Research
Protocol
Name
sortChIC [version without A-tailing]
Version 1
, Hubrecht Institute for Developmental Biology and Stem Cell Research
Samy KefalopoulouHubrecht Institute for Developmental Biology and Stem Cell Research
Protocol
Name
sortChIC in bulk [version without A-tailing]
Version 1
, Hubrecht Institute for Developmental Biology and Stem Cell Research
Samy KefalopoulouHubrecht Institute for Developmental Biology and Stem Cell Research
Protocol
Name
scDam&T-seq [version half volumes]
Version 1
, Hubrecht Institute for Developmental Biology and Stem Cell Research
Samy KefalopoulouHubrecht Institute for Developmental Biology and Stem Cell Research
Protocol
Name
FACS to measure epitopes in CellTrace-multiplexed samples
Version 1
, Hubrecht Institute for Developmental Biology and Stem Cell Research
Samy KefalopoulouHubrecht Institute for Developmental Biology and Stem Cell Research