May 16, 2024
Version 6
DAB Detection of Biocytin Labeled Tissue V.6
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute for Brain Science 2024. DAB Detection of Biocytin Labeled Tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2ly3xkegx9/v6Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 15, 2024
Last Modified: May 16, 2024
Protocol Integer ID: 99893
Keywords: DAB, biocytin, diaminobenzidine, staining, coverslipping, mounting, PF0285, dab detection of biocytin, detection of biocytin, dab detection, process for diaminobenzidine, use with brain slice, brain slice, tissue this protocol, dab, electrophysiological recording, filled cell, national institutes of health, cell,
Abstract
This protocol describes the process for diaminobenzidine (DAB) detection of biocytin filled cells. This protocol is optimized for use with brain slices cut at 350 µm thick, in which cells are first filled with biocytin (i.e., post-electrophysiological recording), fixed in 4% PFA/2.5% glutaraldehyde, and transferred to PBS until ready to stain.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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