Nov 20, 2025
D16SC Recipe 2 for Allobaculum stercoricanis DSM 13633
- wujunfeng 1
- 1Nanjing University of Posts and Telecommunications
Protocol Citation: wujunfeng 2025. D16SC Recipe 2 for Allobaculum stercoricanis DSM 13633. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ld6dqxg5b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 20, 2025
Last Modified: November 20, 2025
Protocol Integer ID: 233035
Keywords: d16sc recipe, allobaculum stercoricanis dsm, deep16sculture ai framework for allobaculum stercoricanis dsm, deep16sculture ai framework, recipe
Abstract
This recipe was generated by our Deep16SCulture AI framework for Allobaculum stercoricanis DSM 13633.
Materials
- Trypticase peptone: 2000 mg
- Yeast extract: 2000 mg
- Glucose: Added 5000 mg
- L-Cysteine HCl x H₂O: 500 mg
- Na-acetate: 1000 mg
- NaCl: 18010 mg
- Vitamin mix (biotin, B₁₂, etc.): Keep as-is (optional)
- Water: 1011 ml
Troubleshooting
Before start
- Add 0.5 g/L sodium thioglycolate as an additional reducing agent.
- Adjust pH to 6.8–7.2 with NaHCO₃.
Notes
Add 0.5 g/L sodium thioglycolate as an additional reducing agent.
Adjust pH to 6.8–7.2 with NaHCO₃.