Nov 20, 2025

Public workspaceD16SC Recipe 1 for Limosilactobacillus mucosae CCUG 43179T

DOI
https://dx.doi.org/10.17504/protocols.io.81wgbwbqogpk/v1
  • wujunfeng 1
  • 1Nanjing University of Posts and Telecommunications.
wujunfeng
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DOI: https://dx.doi.org/10.17504/protocols.io.81wgbwbqogpk/v1
Protocol Citation: wujunfeng 2025. D16SC Recipe 1 for Limosilactobacillus mucosae CCUG 43179T. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbwbqogpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working.
Created: November 20, 2025
Last Modified: November 20, 2025
Protocol Integer ID: 233033
Keywords: d16sc recipe, deep16sculture ai framework for limosilactobacillus, limosilactobacillus, recipe, deep16sculture ai framework
Abstract
This recipe was generated by our Deep16SCulture AI framework for Limosilactobacillus mucosae CCUG 43179T.
Materials
- Columbia agar: 39,000 mg
- Sheep blood: 100 ml
- Water: 1,000 ml
Troubleshooting
Improvements
Added: Glucose (5,000 mg) to support fermentation.
Optional: None (blood is critical).
Notes
Incubate anaerobically at 37°C for 48–72 hours.
Sheep blood provides hemin and growth factors.