Nov 20, 2025
D16SC Recipe 1 for Blautia coccoides JCM 1395
- wujunfeng 1
- 1Nanjing University of Posts and Telecommunications
Protocol Citation: wujunfeng 2025. D16SC Recipe 1 for Blautia coccoides JCM 1395. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov116xovr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 20, 2025
Last Modified: November 20, 2025
Protocol Integer ID: 233045
Keywords: d16sc recipe, deep16sculture ai framework for blautia, jcm 1395 this recipe, blautia, recipe, deep16sculture ai framework
Abstract
This recipe was generated by our Deep16SCulture AI framework for Blautia coccoides JCM 1395.
Guidelines
- Most suitable
- Added: None (already contains all key ingredients).
- Optional: Ethanol, Tween 80.
- Notes: Pre-reduce medium by boiling and cooling under N2/CO2.
Materials
- Beef extract: 5000 mg
- Glucose: 5000 mg
- Peptone: 5000 mg
- Yeast extract: 10,000 mg
- L-Cysteine HCl: 500 mg
- NaHCO3: 400 mg
- Haemin: 5 mg
- Vitamin K1: 0.001 ml
- Resazurin: 1 mg
Optional: Ethanol, Tween 80, CaCl2, MgSO4.
Troubleshooting
Most suitable
Pre-reduce medium by boiling and cooling under N2/CO2.