Nov 20, 2025
D16SC Recipe 1 for Allobaculum stercoricanis DSM 13633
- wujunfeng 1
- 1Nanjing University of Posts and Telecommunications
Protocol Citation: wujunfeng 2025. D16SC Recipe 1 for Allobaculum stercoricanis DSM 13633. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl46nd2go5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 20, 2025
Last Modified: November 20, 2025
Protocol Integer ID: 233041
Keywords: d16sc recipe, allobaculum stercoricanis dsm, deep16sculture ai framework for allobaculum stercoricanis dsm, deep16sculture ai framework, recipe
Abstract
This recipe was generated by our Deep16SCulture AI framework for Allobaculum stercoricanis DSM 13633.
Materials
- Bacto Soytone: 5000 mg
- Casein peptone: 10000 mg
- Glucose: 10000 mg
- Yeast extract: 5000 mg
- L-Cysteine HCl x H2O: 500 mg
- Meat extract: 5000 mg
- MgSO4 x 7 H2O: 220 mg
- NaCl: 5080 mg
- Resazurin: 4 ml (optional)
- Tween 80: 1 ml (optional)
- Water: 990 ml
Troubleshooting
Before start
- Pre-reduce medium by boiling and cooling under anaerobic conditions (N2/CO2 atmosphere).
- Incubate at 37°C in anaerobic jars with gas packs.
Notes
Pre-reduce medium by boiling and cooling under anaerobic conditions (N2/CO2 atmosphere).
Incubate at 37°C in anaerobic jars with gas packs.