CZV8-proto agar 500 mL 300 mLCzapek-Dox broth 22.7 g 13.62 gagar 5 g 3 gsorbitol 91.1 g 54.7 g centrifuged V8 juice 100 mL 60 mLadd H2O to 500 mL 300 mLadjust to pH 6.0 with NaOHautoclave and pour thin plates (approx. 10mL)