Aug 21, 2025

Public workspaceCylinder test in rats

 Forked from Cylinder test in rats
DOI
https://dx.doi.org/10.17504/protocols.io.14egnypmzv5d/v1
  • Eduard Bentea1,
  • María Sanchiz Calvo1,
  • Veerle Baekelandt1
  • 1Katholieke Universiteit Leuven
María Sanchiz Calvo
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DOI: https://dx.doi.org/10.17504/protocols.io.14egnypmzv5d/v1
Protocol Citation: Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt 2025. Cylinder test in rats. protocols.io https://dx.doi.org/10.17504/protocols.io.14egnypmzv5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 24, 2025
Last Modified: August 21, 2025
Protocol Integer ID: 220875
Keywords: ASAPCRN, cylinder test in rat, cylinder test in rats protocol, cylinder test, nigrostriatal dopaminergic pathway, test, rats protocol, animal models with unilateral lesion, rat, asymmetry in forelimb use
Funders Acknowledgements:
ASAP (Aligning Science Across Parkinson's)
Abstract
Protocol for performing the cylinder test in rats. The cylinder test evaluates asymmetry in forelimb use, and can be used in animal models with unilateral lesions of the nigrostriatal dopaminergic pathway.
Troubleshooting
Test
1h
Bring cages to the behavioral room for at least Duration01:00:00 prior to the test for habituation

1h
Place each rat in a glass cylinder (20-cm wide), surrounded by mirrors to allow a full 360 degree view. Videotape from the front for Duration00:05:00 or until the rat performs a minimum of 20 weight-bearing forepaw contacts with the cylinder walls.

5m
Perform blinded analysis from the recorded videos of the number of contralateral forepaw contacts, and express these as a percentage of total forepaw contacts.