Mar 14, 2024

Public workspaceCylinder Test

DOI
https://dx.doi.org/10.17504/protocols.io.5qpvo3jmzv4o/v1
  • Xiaobo Mao1,2,3,4,
  • Ramhari Kumbhar1,2,3,4,
  • Hanseok Ko1,2,4,
  • Valina L. Dawson1,2,4,3,5,6,
  • Ted Dawson1,2,4,3,5,6,7
  • 1Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;
  • 2Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;
  • 3Adrienne Helis Malvin Medical Research Foundation, New Orleans, LA 70130-2685, USA;
  • 4Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA;
  • 5Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA;
  • 6Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;
  • 7Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA
Jacquelyn Haytayan
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DOI: https://dx.doi.org/10.17504/protocols.io.5qpvo3jmzv4o/v1
Protocol Citation: Xiaobo Mao, Ramhari Kumbhar, Hanseok Ko, Valina L. Dawson, Ted Dawson 2024. Cylinder Test. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvo3jmzv4o/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 14, 2024
Last Modified: September 23, 2024
Protocol Integer ID: 96716
Keywords: ASAPCRN, behavior, mouse model, cylinder test the cylinder test, cylinder test, locomotor asymmetry, spontaneous movement in rodent model, rodent model, spontaneous movement
Funders Acknowledgements:
Aligning Science for Parkinson's
Grant ID: 020608
Abstract
The Cylinder test is used to evaluate locomotor asymmetry and spontaneous movement in rodent models.
Materials
  • Small transparent cylinder (height, 15.5 cm; diameter, 12.7 cm)
  • Video recorder
Troubleshooting
Set up small, transparent cylinder in front of video recorder.
Place mouse in cylinder and record spontaneous activity for 10 min.
Clean cylinder in between mice.
For all recordings, view in slow motion and count the number of forepaw touches to the cylinder walls, rears, and grooming bouts.