Oct 12, 2023

Public workspaceCylinder Test

DOI
https://dx.doi.org/10.17504/protocols.io.81wgbxxpnlpk/v1
  • Roberta Marongiu1,2,
  • Sabina Marciano3
  • 1Department of Neurological Surgery, Weill Cornell Medical College, New York, NY 10065;
  • 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA;
  • 3Weill Cornell medicine
Jacquelyn Haytayan
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DOI: https://dx.doi.org/10.17504/protocols.io.81wgbxxpnlpk/v1
Protocol Citation: Roberta Marongiu, Sabina Marciano 2023. Cylinder Test. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbxxpnlpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 14, 2023
Last Modified: September 23, 2024
Protocol Integer ID: 86457
Keywords: ASAPCRN, locomotor asymmetry in rodent model, locomotor asymmetry, cylinder test the cylinder test, cylinder test, rodent model, cylinder, test
Abstract
The Cylinder test is used to evaluate locomotor asymmetry in rodent models

Materials
  • Glass cylinder 20 cm in diameter 30 cm high
  • Angled mirror
  • 70% ethanol for cleaning between each session
Troubleshooting
Place the mouse in the transparent cylinder for Duration00:05:00

5m
Place an angled mirror at the back of the cylinder to allow observation of behavior from every angle
Record mouse behavior for Duration00:05:00

Main outcome measures: Left/Right contacts, Number of rears, L/R hind-paw contacts, Total time rearing, Time on the ground
5m