Feb 13, 2025
Version 2
CUMC-TMC-10X-Chromium-Next GEM Single Cell Multiome ATAC + Gene Expression V.2
- Shaunice Grier1
- 1Columbia University
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Shaunice Grier 2025. CUMC-TMC-10X-Chromium-Next GEM Single Cell Multiome ATAC + Gene Expression. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvwb45dvmk/v2Version created by Shaunice Grier
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 12, 2025
Last Modified: February 13, 2025
Protocol Integer ID: 120146
Keywords: chromium next gem single cell multiome atac, next gem single cell multiome atac, gene expression post mortem human central, 10x genomic, post mortem human central nervous system tissue, atac, gene expression user guide, gene expression, genomic, same tissue block, nervous system tissue, rna, post mortem human central, nuclei, gene, same balance of cell type
Abstract
Post Mortem Human Central Nervous System tissue is sectioned at 50 µm thickness from the same tissue block and plane from previous Visium ST experiments. This method is used to capture the same balance of cell types and cortical layers across both techniques for each subject. Nuclei is isolated from frozen post-mortem CNS samples. RNA/ATAC sequencing is performed according to the 10X Genomics’ Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User Guide (CG000338 Rev F).
Attachments
Guidelines
Protocol needs prior approval by the users' Institutional Review Board (IRB) or equivalent ethics committee(s)
Troubleshooting