we generated five clonal, axenic strains of Symbiodinium. These strains were assigned to clades A (two strains), B, E, and F based on their chloroplast 23S rDNAsequences. Growth studies in liquid cultures showed that the clade B strain and one of the clade A strains were able to grow photoautotrophically (in light withno fixed carbon), mixotrophically (in light with fixed carbon), or heterotrophically (in dark with fixed carbon). Here this protocol describes the steps of culturing SSB01 in different types of medium as an example.