Sep 04, 2020

Public workspaceCulturing Chlamydomonas reinhardtii

DOI
dx.doi.org/10.17504/protocols.io.bkrskv6e
  • 1Ronin Institute
Joao Vitor Molino
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DOI: dx.doi.org/10.17504/protocols.io.bkrskv6e
Protocol Citation: Joao Vitor Molino 2020. Culturing Chlamydomonas reinhardtii. protocols.io https://dx.doi.org/10.17504/protocols.io.bkrskv6e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 04, 2020
Last Modified: September 04, 2020
Protocol Integer ID: 41490
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Abstract
This protocols describe the steps required for the growth of Chlamydomonas reinhardtii in liquid media.

Guidelines
All steps described in this protocol are intended to be conducted in a research laboratory. Follow aseptic procedures.
Material
Material
  • TAP media or other (How to prepare TAP media here)
  • Erlenmeyer flask
  • Orbital shaker
  • Light source
Inocullum
Inocullum
*Inoculation can be perfomed by scraping an agar plate containing Chlamydomonas reinhardtii or from another liquid culture
  1. Innoculate the cells in an erlenmeyer containing media (Ex: Amount100 mL Erlenmeyer flask containing Amount50 mL of TAP media or Amount500 mL Erlenmeyer flask containing Amount250 mL )
  2. Place the flask in a shaker at Shaker150 rpm, 25°C with Amount1 cm of orbit and with Amount80 μmol/m2s of incident white light ( Amount60 μmol/m2s to Amount120 μmol/m2s works)

Time to stationary phase depends on initial innoculum.