As the interest to understand melanized fungi becomes more of a focus due to pathological diseases, there needs to be a clearer method to isolate and identify the fungi from their surroundings. Culture independent analysis has helped bloom scientist understandings of the vast quantities of microorganisms around us, but culturing and identifying has always been a struggle. We must look into utilizing different microbiological techniques to help better understand, isolate, and to apply Koch\u2019s postulates to prove they are the cause of said disease. Growing and isolating fungi has always been an issue especially if the fungi in question is a slow growing fungi, where fast growing fungi or other microorganisms can grow and surpass the field of view and compete with the slow growing fungi. A series of dilutions, antibiotics and oligotrophic media can all counteract the issues to provide you with a clear window to help isolate your fungi in question. There is still a need to verify that living strains of organisms are actually present in the environment. One approach is to use \u201cculture dependent\u201d method to obtain strains of organisms present in the biocrust. This is achieved by plating biocrust soils onto microbiological media in order to further isolate life strains. Serial dilution is used to isolate the fungi from the soil. This method involves making a soil slurry by diluting the soil sample with increasing higher proportion of water or media in order to get a low starting concentration of starting spores or material growing on the Petri dishes.