Feb 15, 2022
CTAB DNA Extraction of Plant Tissue
This protocol is a draft, published without a DOI.
- 1Realizing Increased Photosynthetic Efficiency (RIPE)
- GEGC lab UIUC
- UIUC Long Lab
Protocol Citation: Lynn Doran 2022. CTAB DNA Extraction of Plant Tissue. protocols.io https://protocols.io/view/ctab-dna-extraction-of-plant-tissue-b44kqyuw
Manuscript citation:
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: February 15, 2022
Last Modified: July 11, 2023
Protocol Integer ID: 58220
Keywords: DNA, DNA Extraction, CTAB
Abstract
CTAB DNA extraction using chloroform/isoamyl alcohol wash, precipitation in isopropanol, and ethanol rinses for leaf tissue.
Attachments
Image Attribution
Microsoft Office Clipart Library
Safety warnings
- This protocol uses chemical fume hoods and centrifuges. Understand how to safely and appropriately use them before performing the protocol.
- Some of the chemicals used in this protocol pose serious health risks. Please read all manufacturer safety data sheets before handling. UIUC personnel performing this protocol should be current on "Laboratory Safety", "Chemical Safety- An Introduction", and "Chemical Spills" Division of Research Safety training modules before performing this protocol.