Jul 17, 2020
Version 1
Croprological flotation techniques Tibor-Kassai, 1998 V.1
- Javier Antonio Benavides-Montaño1,
- Javier Antonio Benavides1,
- ptzarater@unal.edu.co1
- 1Universidad Nacional de Colombia
External link: https://doi.org/10.1371/journal.pntd.0008734
Protocol Citation: Javier Antonio Benavides-Montaño, Javier Antonio Benavides, ptzarater@unal.edu.co 2020. Croprological flotation techniques Tibor-Kassai, 1998. protocols.io https://dx.doi.org/10.17504/protocols.io.bipkkdkw
Manuscript citation:
Peña-Quistial MG, Benavides-Montaño JA, Duque NJR, Benavides-Montaño GA (2020) Prevalence and associated risk factors of Intestinal parasites in rural high-mountain communities of the Valle del Cauca—Colombia. PLoS Negl Trop Dis 14(10): e0008734. doi: 10.1371/journal.pntd.0008734
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 16, 2020
Last Modified: July 17, 2020
Protocol Integer ID: 39372
Disclaimer
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK
The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Prepare a Sheather's Sucrose Solution. Density = 1.20-1.27. Preparation: dissolve over indirect heat (to avoid caramelization) approximately 500 g of granulated sugar (sucrose) in 1 liter of boiling water; add 6 ml of 37% formaldehyde or crystallized phenol to prevent microbial growth (mold).
Immerse 3-5 g of feces in water for 30 minutes; dissolve the stool balls. using a glass rod or an electric homogenizer until a semi-solid suspension is obtained.
Deposit the mixture in a centrifuge tube and centrifuge at 1500 rpm for 2 minutes, subsequently discarding the supernatant
Re-suspend the sediment completely by shaking
Fill the tube with one of the flotation solutions. mix the contents, covering the tube with your thumb and inverting it several times; do not shake, avoid the formation of air bubbles
Centrifuge again at 1500 rpm for 2 minutes
Place the drop where the eggs are on a slide, lightly pressing the glass rod or the handle on it; add a drop of water, place a coverslip and examine the entire preparation under the microscope using the 10X objective; using the 40X objective to appreciate in detail the morphology of the eggs