This procedure describes the steps required to create diffusion tensor images (DTI) from data from the MRI scanner. The following programs are required to go through the protocol:MRtrix3 TORTOISEITKsnapNote: If you are working from a Windows computer, you will need to follow X2Go Client Set-Up (dx.doi.org/10.17504/protocols.io.6tvhen6) before you can begin this protocol.