Oct 27, 2021

Public workspaceCPMU V.1

DOI
dx.doi.org/10.17504/protocols.io.n92ld96dng5b/v1
  • 1Leiden University
  • BYOC
Bjørn Peare Bartholdy
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DOI: dx.doi.org/10.17504/protocols.io.n92ld96dng5b/v1
Protocol Citation: Bjorn Bartholdy, a.g.henry 2021. CPMU. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ld96dng5b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: July 01, 2021
Last Modified: October 27, 2021
Protocol Integer ID: 51183
Abstract
Mineralising solution for the oral biofilm. From Sissons et al. 1991.
Materials
Chemicals
dH2O
CaCl2
NaH2PO4
MgCl
Urea

Equipment
1000 mL beaker
Positive flow fumehood (or other starch-free environment)
Protocol materials
ReagentMagnesium ChlorideFisher ScientificCatalog #AC223210010
ReagentSodium Phosphate monobasic
ReagentSodium FluorophosphateMerck MilliporeSigma (Sigma-Aldrich)Catalog #344443
ReagentUreaP212121
ReagentCalcium Chloride
Before start
This solution MUST be prepared in a sterile and starch-free environment.
Add Amount300 mL distilled (or deionized) dH2O to a Amount1000 mL beaker, with stirring and heat Temperature60 °C
Add:

- Amount1.55 g ReagentCalcium ChlorideSigma Aldrich
- Amount1.44 g ReagentSodium Phosphate monobasicSigma Aldrich
- Amount0.72 g ReagentSodium FluorophosphateSigma AldrichCatalog #344443
- Amount0.08 g ReagentMagnesium ChlorideSigma AldrichCatalog #AC223210010
- Amount30 g ReagentUreaSigma Aldrich

Add the remaining Amount700 mL and keep stirring until precipitate has completely dissolved
Store in fridge at Temperature4 °C