Sep 07, 2020
Version 1
CoVIDScandx V.1
- 1The Lancaster University
Protocol Citation: Muhammad Munir 2020. CoVIDScandx. protocols.io https://dx.doi.org/10.17504/protocols.io.bkzikx4e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 07, 2020
Last Modified: September 08, 2020
Protocol Integer ID: 41738
Add smart lysis buffer with 1:1 ratio in the VTM sample
Heat for 68C for 8 minutes and then 95C for 10 minutes
Cool and take 3 ul to colorimetric LAMP master mix (NB: M1800)
Vortex reaction mix and centrifuge to collect material
Pipet 24 μl per reaction into desired reaction vessels and add 1 µl of sample. Mix by vortexing or by pipetting if using a plate or similar vessel, centrifuge to collect if necessary. Check that reaction solutions have a bright pink colour, which indicates initial high pH required for successful pH-LAMP reaction.
Incubate at 65°C for 30 minutes.
Cool on ice for 2 minutes
Add 5 ul of the LAMP reaction with the sample buffer
Load to the LFD strip and let is absorbe
Visualize the bands for internal control and SARS-CoV-2
Scan with mReader to record results