Sep 07, 2020
Version 2
covid 19 indirect detection thru rise of 100nm filter air resistance V.2
- Coronavirus Method Development Community
- XPRIZE Rapid Covid Testing
Protocol Citation: Ladislav Nevery 2020. covid 19 indirect detection thru rise of 100nm filter air resistance . protocols.io https://dx.doi.org/10.17504/protocols.io.bkznkx5eVersion created by Ladislav Nevery
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: September 07, 2020
Last Modified: September 07, 2020
Protocol Integer ID: 41742
Keywords: covid, pressure, test,
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Abstract
By passing saliva sample thru 200nm filter to remove particles larger than 120nm covid 19 virus particles and concentrating resulting fluid to very small 1 mm2 area of 100nm filter. Pores in this small area of100nm filter should be clogged up fast raising filter air resistance. Due to high virus density 5.2 log10 ml saliva of infected person should clogg filter much faster than healthy person. Test is setup in such way that act of compressor automatically turning off after reaching threshold PSI value will indicate high count of particles larger than 100nm of being present in measured filter area.
Attachments
Materials
MATERIALS
pragopor 10pragochema
pragopor 8pragochema
3d filament
rubber seal 24x20x2
STEP MATERIALS
pragopor 10pragochema
rubber seal 24x20x2
Target2™ Nylon Syringe Filter, 0.2µm, 30mmThermo FisherCatalog #F2500-2
Protocol materials
Target2™ Nylon Syringe Filter, 0.2µm, 30mmThermo FisherCatalog #F2500-2
pragopor 10pragochema
pragopor 8pragochema
3d filament
rubber seal 24x20x2
pragopor 10pragochema
rubber seal 24x20x2
rubber seal 24x20x2
Target2™ Nylon Syringe Filter, 0.2µm, 30mmThermo FisherCatalog #F2500-2
pragopor 10pragochema
3d print all required parts.
insert any 100nm 24mm filter like for example
pragopor 10pragochema
between blueparts and close with red cup
rubber seal 24x20x2
.Place any comon commercial 200nm filter between syringe and red syringe input port. colors are on attached image above
Target2™ Nylon Syringe Filter, 0.2µm, 30mmThermo FisherCatalog #F2500-2
5m
pass sample thru both 200nm and 100nm filter by applying manual pressure on syringe.
5m
now that 1 mm2 area of 100nm filter has enough pores clogged up with median 120nm sized virus particles. replace red syringe cap with green compressor input cap.Use any compressor wich can turn itself offwhen reaching set pressure. I used
Equipment
Xiaomi Portable Compressor
NAME
Portable Compressor
TYPE
Xiaomi
BRAND
00000000
SKU
LINK
set compressor to turn off after reaching threshold amount of psi and turn the compressor on.
Expected result
On sample without covid 19 virus threshold psi should not be reached
. If no compressor with proper psi sensitivity is available then monitor pressure in concentration chamber by any external sensitive manometer
5m