Illumina\u00ae short-read DNA sequencing has become an integral tool in biology for genome-wide studies. Offering accurate base-pair resolution at the most competitive price, the technology has become widespread. However, the generation of multiplexed DNA libraries remains costly and cumbersome. Here, we present a streamlined cost-conscious protocol for generating multiplexed short read DNA libraries using a transposase from Illumina\u00ae. By implementing small volumes that use 1\/25th the amount of transposase compared to Illumina\u00ae NexteraTM protocols, the cost of library preparation can be significantly reduced, by 1\/10th or more. Furthermore, we optimised the protocol to minimise carboxylate bead-based cleanups between steps, further reducing cost, time and DNA input. By developing our own indicies to multiplex nine 96-well plates, up to 864 samples can be placed on a single flow cell. This enables efficient usage of monolithic sequencing platforms that can offer over three terabases of sequencing per flow cell.