This protocol is designed to work with coral host tissue slurry that has been processed by airbrushing to remove the tissue from the skeleton and after removal of symbiont cells and homoginization. This protocol will allow quantification of lipid concentrations of samples using 96-well plates for rapid assessment. This protocol was adapted from Folch et al 19571 and Cheng et al 20112. For calculation of lipid concentrations, follow the directions presented in the materials section or use the custom Markdown file that can be found on my GitHub (LipidAssay_96wellplate).Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497-509. PMID: 13428781.Cheng YS, Zheng Y, VanderGheynst JS. Rapid quantitative analysis of lipids using a colorimetric method in a microplate format. Lipids. 2011 Jan;46(1):95-103. doi: 10.1007\/s11745-010-3494-0. Epub 2010 Nov 11. PMID: 21069472.