Measures iron from 0.4 - 20 nmol\/83366) to quantify the levels of iron in cell culture and meat samples. Iron dissociates from its carrier protein in the presence of acidic buffer. When reduced to Fe2+, ions will react with Ferene S to produce a complex with absorbance at 593 nm. Cu2+ interference is blocked with a chelate chemical included in the buffer. This protocol is designed to measure iron from 0.4 - 20 nmol\/50 \u03bcL sample or 8 \u03bcM - 400 \u03bcM.